Fig. 2: Stimulated emission depletion (STED) micrographs and nearest neighbor analysis of mitochondria, Bcl-xL, and Bax in high glucose (HG)-treated proximal tubular cells (PTCs). | Cell Death & Disease

Fig. 2: Stimulated emission depletion (STED) micrographs and nearest neighbor analysis of mitochondria, Bcl-xL, and Bax in high glucose (HG)-treated proximal tubular cells (PTCs).

From: Super-resolution microscopy reveals that Na+/K+-ATPase signaling protects against glucose-induced apoptosis by deactivating Bad

Fig. 2

A Representative images showing the distributions of Bax (green) and Bcl-xL (magenta) together with mitochondria (gray). The top row shows untreated (5.6 mM glucose) cells. The second and third rows show cells treated with HG for 4 and 6 h, respectively. B Nearest neighbor analysis of interactions based on the accumulated statistics for distances between Bcl-xL and Bax measured by super-resolution STED microscopy. Bars show the fractions of proteins with a separating distance indicated at x axis. The <50 nm distance fraction (red) is considered indicative of a molecular interaction. N = number of micrographs from different cells obtained from 3 or more cell culture preparations; 31 in control, 36 in 4 h HG, and 28 in 6 h HG.

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