Fig. 2: Arid1a-deficient mice are more susceptible to VSV infection. | Cell Death & Disease

Fig. 2: Arid1a-deficient mice are more susceptible to VSV infection.

From: Chromatin remodeler ARID1A binds IRF3 to selectively induce antiviral interferon production in macrophages

Fig. 2

A ELISA analysis of IFN-I in the serum of Arid1a f/f Lyz-Cre− and Arid1a f/f Lyz-Cre+ mice infected with VSV (5 × 107 pfu/g, n = 3 per group) at indicated times. B ELISA analysis of TNF-α and IL-6 in the serum of mice treated as in (A). (C) qPCR analysis of Ifnb expression in spleen, lung, liver and PMs of mice treated as in (A). D TCID50 assay of VSV load in spleen, lung, and liver of Arid1a f/f Lyz-Cre− and Arid1a f/f Lyz-Cre+ mice 18 h after VSV infection (5 × 107 pfu/g, n = 3 per group). E HE staining of the lung tissue from mice treated as (D) (scale bar: 100 μm). F Survival curve analysis of Arid1a f/f Lyz-Cre− and Arid1a f/f Lyz-Cre+ mice intravenously injected with VSV (1 × 108 pfu/g, n = 7 per group). Kaplan–Meier survival curves were generated and analyzed for statistical significance with GraphPad Prism 5.0. Error bars represent s.d. Student’s t-test. *P < 0.05, **P < 0.01. All data are representative of three independent experiments with three biological replicates. (A–D: mean ± s.d.).

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