Fig. 4: ARID1A interacts with NSD2 to promote methylation of Ifn-I promoters. | Cell Death & Disease

Fig. 4: ARID1A interacts with NSD2 to promote methylation of Ifn-I promoters.

From: Chromatin remodeler ARID1A binds IRF3 to selectively induce antiviral interferon production in macrophages

Fig. 4

A Co-IP analysis of the interaction between endogenous ARID1A and NSD2 in macrophages. B Immunofluorescence of ARID1A and NSD2 localization in macrophages (scale bar: 10 μm). C Co-IP analysis of the interaction between tagged ARID1A and tagged wild type NSD2 or indicated NSD2 mutants in HEK293T cells. D Co-IP analysis of the interaction between tagged IRF3 and tagged wild type NSD2 or indicated NSD2 mutants in HEK293T cells. E–F ChIP assay of H3K4Me3 and H3K36Me2 in the Ifnb (E) and Ifnα (F) promoter regions of Arid1a f/f Lyz-Cre− and Arid1a f/f Lyz-Cre+ BMDMs with VSV infection (MOI, 1) at the indicated times. G, H ChIP assay of H3K4Me3 and H3K36Me2 in the Tnfα (G) and Il6 (H) promoter regions of cells as in (E–F). All data are representative of three independent experiments with three biological replicates. *P < 0.05, **P < 0.01. (E–H: mean ± s.d.).

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