Fig. 5: Inhibition of Nsd2 restrains IFN-I production and histone methylation upon virus infection. | Cell Death & Disease

Fig. 5: Inhibition of Nsd2 restrains IFN-I production and histone methylation upon virus infection.

From: Chromatin remodeler ARID1A binds IRF3 to selectively induce antiviral interferon production in macrophages

Fig. 5

A, B qPCR analysis of Ifn-I (A) and ELISA analysis of IFN-I (B) expression in PMs silenced with two specific Nsd2 siRNAs or NC siRNA in response to VSV (MOI, 1) infection at the indicated times. C, D qPCR analysis of Ifn-I (C) and ELISA analysis of IFN-I (D) expression in Nsd2+/+ or Nsd2+/− RAW 264.7 cells infected with VSV (MOI, 1) at the indicated times. E, F ChIP assay of H3K4Me3 and H3K36Me2 in the Ifnb (E) and Ifnα (F) promoter regions of Nsd2+/+ and Nsd2+/− RAW264.7 cells infected with VSV (MOI, 1) at the indicated times. *P < 0.05, **P < 0.01. All data are representative of three independent experiments. (A–F: mean ± s.d.).

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