Fig. 6: NSD2 promotes IFN-I production by mediating histone methylation of Ifn-I promoters. | Cell Death & Disease

Fig. 6: NSD2 promotes IFN-I production by mediating histone methylation of Ifn-I promoters.

From: Chromatin remodeler ARID1A binds IRF3 to selectively induce antiviral interferon production in macrophages

Fig. 6

A, B qPCR analysis of Ifn-I (A) and ELISA analysis of IFN-I (B) expression in Arid1a f/f Lyz-Cre- and Arid1a f/f Lyz-Cre+ BMDMs transfected with wild type Arid1a and Arid1a-ΔARID construct and then infected with VSV (MOI, 1) at the indicated times, mock served as control. C, D qPCR analysis of Ifn-I (C) and ELISA analysis of IFN-I (D) expression in Arid1a f/f Lyz-Cre− and Arid1a f/f Lyz-Cre+ BMDMs transfected with wild type Nsd2 or Nsd2-ΔSET construct and then infected with VSV (MOI, 1) at the indicated times, mock served as control. E, F ChIP assay of H3K4Me3 and H3K36Me2 in the Ifnb (E) or Ifnα (F) promoter regions of Arid1a f/f Lyz-Cre− and Arid1a f/f Lyz-Cre+ BMDMs transfected with plasmids as in (C–D) in response to VSV (MOI, 1) infection at the indicated times. Error bars represent s.d. Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001. All data are representative of three independent experiments with three biological replicates. (A–F: mean ± s.d.).

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