Fig. 1: ALCAP2 inhibits the proliferation of LUAD cells. | Cell Death & Disease

Fig. 1: ALCAP2 inhibits the proliferation of LUAD cells.

From: ALCAP2 inhibits lung adenocarcinoma cell proliferation, migration and invasion via the ubiquitination of β-catenin by upregulating the E3 ligase NEDD4L

Fig. 1

A Chemical structure of ALCAP2. B–E The viability of A549, H1299, and HCC827 cells and bronchial epithelial BEAS-2B cells was determined by MTT assay after the cells treated with ALCAP2 at the indicated concentrations. The IC50 of ALCAP2 for each cell line was calculated according to a cell viability value. F, G An EdU staining assay was performed to determine the proliferation capacity of A549 and H1299 cells treated with or without ALCAP2. EdU-positive ratios are shown. Scale bar, 2 mm. H, I Representative images of the results of the clonogenic analysis of A549 and H1299 cell proliferation after treatment with or without ALCAP2. Student’s t test was used for statistical analysis and data were presented as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.

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