Fig. 4: ALCAP2 suppresses the total and nuclear protein levels of β-catenin. | Cell Death & Disease

Fig. 4: ALCAP2 suppresses the total and nuclear protein levels of β-catenin.

From: ALCAP2 inhibits lung adenocarcinoma cell proliferation, migration and invasion via the ubiquitination of β-catenin by upregulating the E3 ligase NEDD4L

Fig. 4

A–C Heat map of differentially expressed mRNAs in the treatment and control groups. A total of 592 genes were identified [false discovery rate (FDR) <0.05 and absolute fold change (FC absolute) >0.585], among which there were 373 were upregulated and 219 were downregulated. Then, we performed a GO enrichment analysis of these genes. D A549 and H1299 cells were treated with different concentration of ALCAP2 for 24 h, cells were lysed with 1 ml of RIPA buffer for western blot. E A549 were treated with 1.0 μM ALCAP2 and H1299 treated with 0.6 μM ALCAP2 for different time, then cells were lysed with 1 ml of RIPA buffer for western blot. F A549 and H1299 cells treated with or without ALCAP2 were subjected to cellular fractionation followed by western blotting for detecting β-catenin. Tubulin and Lamin B1 are endogenous markers of cytosolic and nuclear proteins (NE: Nuclear, Non-NE: Cytoplasmic). G Immunofluorescence staining of β-catenin after A549 and H1299 cells were treated with or without ALCAP2.

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