Fig. 5: ALCAP2 promotes β-catenin ubiquitination by increasing the expression of NEDD4L. | Cell Death & Disease

Fig. 5: ALCAP2 promotes β-catenin ubiquitination by increasing the expression of NEDD4L.

From: ALCAP2 inhibits lung adenocarcinoma cell proliferation, migration and invasion via the ubiquitination of β-catenin by upregulating the E3 ligase NEDD4L

Fig. 5

A The mRNA level of β-catenin was not significantly different after treatment with ALCAP2. B The degradation of β-catenin mediated by CHX. H1299 cells treated with or without ALCAP2 were seeded into six-well plates. After 24 h, the cells were treated with 100 µg/ml CHX. Cell lysates were prepared for western blot analysis. C Three databases include upregulated genes in the RNA-Seq results [absolute fold change (FC absolute) >0.1 and p < 0.05], definitive human E3 ligases and E3 ligases related to β-catenin via UbiBrowser, were used to predict the E3 ligases of β-catenin. D Protein levels of NEDD4L were increased after H1299 cells were treated with ALCAP2. E NEDD4L downregulation increased β-catenin protein expression. F The co-immunoprecipitation assay proved the association between β-catenin and NEDD4L. G The co-immunoprecipitation assay proved that NEDD4L can promote the binding of β-catenin and Ub. H Co-immunoprecipitation of β-catenin and Ub showed that ALCAP2 promoted the binding of β-catenin and Ub, and this effect was reversed in si-NEDD4L-expressing cells.

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