Fig. 5: High-throughput drug screen against cellular morphology via cell painting identifies compounds able to revert aberrant line-specific morphological characteristics.
From: Precision genetic cellular models identify therapies protective against ER stress
A Cell painting images of parental RPE-1 cells. Cells were plated in 384-well tissue culture plate and stained with MitoTracker Red (mitochondrial stain), ConcanavalinA-488 (ER stain), phalloidin-547 (actin stain), and DAPI (nuclei) as described in the “Materials and methods” section. B Representative cell painting images of CDDG and CDG lines. C, D Hierarchical clustering and principal component analyses of extracted morphological features distinguishes CDG and CDDG cell lines from parental RPE-1 cells. E Screening workflow for selection of candidate compounds. F Representative scatter plots of primary screen results for each genotype. Each dot represents one well. For RPE-1 and vehicle treated isogenic CDG and CDDG cell lines, N = 56 replicate wells. For compound treatments, each compound was tested in N = 1 well. G Venn diagram comparing the number and overlap of compounds affecting each CDG/CDDG phenotype.
