Fig. 5: HDAC2 knockout impaired tumor growth and PD–L1 production in vivo. | Cell Death & Disease

Fig. 5: HDAC2 knockout impaired tumor growth and PD–L1 production in vivo.

From: Histone deacetylase 2 knockout suppresses immune escape of triple-negative breast cancer cells via downregulating PD-L1 expression

Fig. 5

A Representative whole-body luminescent images of mice taken at different times after WT and HDAC2-KO cells were intravenously injected into Balb/c mice. B Average lung signal intensities of five mice-injected cells. The measurements were performed at the indicated time points (n = 5). C, D The mice were sacrificed at the 13th day after cell injection, and tumor growth was monitored by gross morphology, H&E staining (C), and lung weight (D, n = 5). E, F Similar to A and B, but 3 × 106 cells were implanted into the breast fat pad of Balb/c mice. G Tumor volumes were calculated every 3–4 days after tumor cells’ implantation (n = 5). H, I Similar to C and D, but the mice were sacrificed at 33 days after tumor cells’ inoculation (n = 5). J PD-L1 expression was determined in tumors by flow cytometry (n = 5).

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