Fig. 1: High hsa_circ_0007813 expression predicted unfavorable prognosis in bladder cancer patients. | Cell Death & Disease

Fig. 1: High hsa_circ_0007813 expression predicted unfavorable prognosis in bladder cancer patients.

From: Autophagy-associated circular RNA hsa_circ_0007813 modulates human bladder cancer progression via hsa-miR-361-3p/IGF2R regulation

Fig. 1

A Heatmap illustrated expression patterns of differentially expressed circRNAs between two bladder cancer tissues (T1 and T2) and their paired adjacent normal tissues (N1 and N2). B Relative hsa_circ_0007813 expression was upregulated in five bladder transitional cell carcinoma cell lines (RT4, UM-UC-3, T24, 5637, and J82), comparing with immortalized uroepithelium cell line SV-HUC-1 (N = 3, Kruskal–Wallis test). C Total cell RNA from T24 and UM-UC-3 was treated with or without RNase R. Expression level of hsa_circ_0007813 and its linear form was measured by qRT-PCR (N = 3, Kruskal–Wallis test). D Diagram of the back-splicing site of hsa_circ_0007813. E FISH for hsa_circ_0007813 in TMA showed that expression of hsa_circ_0007813 was higher in tumor tissues (N = 90, Wilcoxon matched-pairs signed-rank test). F Kaplan–Meier survival plot for bladder cancer patients’ overall survival, grouped by median hsa_circ_0007813 expression level (N = 90, Logrank test). G Representative areas of FISH in TMA were selected to show hsa_circ_0007813 expression between paired tumor and normal samples (Green: hsa_circ_0007813, Blue: DAPI, scale bars represent 1000 μm). All error bars in the figures indicated the standard deviation of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.

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