Fig. 5: CircRNA hsa_circ_0007813 regulated autophagy through hsa-miR-361-3p/IGF2R. | Cell Death & Disease

Fig. 5: CircRNA hsa_circ_0007813 regulated autophagy through hsa-miR-361-3p/IGF2R.

From: Autophagy-associated circular RNA hsa_circ_0007813 modulates human bladder cancer progression via hsa-miR-361-3p/IGF2R regulation

Fig. 5

A Immunocytochemical analysis of autophagosomes in UM-UC-3 cells transfected with siRNA (siCirc) or control siRNA (siCtrl). B At 48 h after siRNA transfection, UM-UC-3 cells were treated with Bafilomycin A1 (Baf A1) 50 nM or DMSO for 24 h. Western blot analysis showed the expression level of SQSTM/p62, LC3-I, and LC3-II. GAPDH was used as loading controls (Left panel). Quantitative analysis was conducted (Right panel, N = 3, Kruskal–Wallis test). C Immunocytochemical analysis of autophagosomes and lysosomes in T24 and UM-UC-3 cells transfected with siRNA (siCirc) or control siRNA (siCtrl). Anti-LC3 and anti-LAMP1 antibodies were used to mark autophagosomes (Green) and lysosomes (Red), respectively. D Immunocytochemical analysis of autophagosomes and lysosomes in T24 and UM-UC-3 cells transfected with hsa_circ_0007813 overexpression vectors (VectCircOE) or control vectors (VectCtrl). Anti-LC3 and anti-LAMP1 antibodies were used to mark autophagosomes (Green) and lysosomes (Red), respectively. The RNA expression profiles of IGF2R were measured by qRT-PCR in (E) hsa_circ_0007813 knockdown and overexpression cells (N = 3, Kruskal–Wallis test), as well as (F) hsa-miR-361-3p downregulated and upregulated cells (N = 3, Kruskal–Wallis test). All error bars in the figures indicated the standard deviation of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.

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