Fig. 1: LINC00671 is a novel LDHA negatively-regulated LncRNA and correlated with good clinical prognosis.

A Schematic of the screening strategy to identify lncRNAs that are downregulated in TC and negatively correlated with LDHA. B Pearson correlation analysis of the expression of LINC00671 and LDHA in TC tissue (http://starbase.sysu.edu.cn). C The expression of LINC00671 in TC patients and normal controls demonstrated by TNM plot under the item of compare tumor and normal section (https://www.tnmplot.com/). D Kaplan–Meier analysis of the overall survival rate (log-rank test, two sides) of TC patients with low (n = 361) or high (n = 310) expression of LINC00671 (http://kmplot.com/analysis/). E The protein levels and mRNA levels of LDHA in TPC-1 cells transfected with empty vector, LINC00298, LINC00671, and LINC01587 expression vectors. Orange bars show the levels of transfected LncRNAs including LINC00671. F The expression levels of LDHA and LINC00671 in TPC-1 cells (left panel) and BCPAP (right panel) transfected with scrambled and LINC00671 smart pool of silencers. The data are expressed as mean ± standard error. G The expression of LINC00671 in TPC-1 and BCPAP cells was detected by qRT-PCR. The separation of the nucleus and cytoplasm was estimated by western blot. Lamin A/C is used as a nuclear marker and tubulin is used as a cytoplasmic marker. H Subcellular localization of LINC00671 (red) in TPC-1 and BCPAP cells by combining RNA FISH and immunofluorescence. The nucleus was stained with DAPI (blue). Scale bar = 10 μm. All values shown are mean ± SD of triplicate measurements and have been repeated three times with similar results (**P < 0.01 versus corresponding control LDHA. ^##P < 0.01 versus corresponding control of lncRNAs).