Fig. 1: Generation and verification of SOX10-knockout hiPSCs. | Cell Death & Disease

Fig. 1: Generation and verification of SOX10-knockout hiPSCs.

From: SOX10 ablation severely impairs the generation of postmigratory neural crest from human pluripotent stem cells

Fig. 1

A Schematic diagram of sgRNAs targeting at exons 1 and 2 of the human SOX10 locus. B Detection of sgRNA:Cas9-mediated cleavage of SOX10 by PCR and T7EN1 cleavage assay (M, DNA marker). C Sanger sequencing was performed on PCR products amplifed from SOX10-knockout hiPSCs. Deletions (−). N/N indicates positive colonies out of total sequenced. WT wild-type sample, KO1 and KO2 the two mutant samples. D The protein expression level of SOX10 in WT and KO cells was detected by western blotting. E The protein expression level of SOX10 in WT and KO cells was further confirmed by immunofluorescence assay. Scale bar, 50 μm. F Cell morphology was observed by phase-contrast microscope. Scale bar, 250 μm.

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