Fig. 4: Characterization of day 7 differentiated cells in WT and KO groups.

A Expression of migrating neural crest markers SOX10/p75 in day 7 differentiated cells in WT and KO groups were detected by immunostaining. Scale bar, 50 μm. B Expression of migrating neural crest markers p75/HNK1 in day 7 differentiated cells in WT and KO groups were detected by immunostaining. Scale bar, 50 μm. C The mRNA level of migrating neural crest markers (SOX10, p75, HNK1) in day 7 differentiated cells was detected by qRT-PCR. n = 3 biological replicates. Columns represent the mean ± SD. **p < 0.01, ***p < 0.001.