Fig. 8: Neuronal and Schwann cell differentiation of NCSCs derived from WT and KO hiPSCs. | Cell Death & Disease

Fig. 8: Neuronal and Schwann cell differentiation of NCSCs derived from WT and KO hiPSCs.

From: SOX10 ablation severely impairs the generation of postmigratory neural crest from human pluripotent stem cells

Fig. 8

A PRPH+ and TUJ1+ neurons were detected by immunofluorescence. Scale bar, 50 μm. B The ratio of PRPH+ and TUJ1+ neurons were counted and compared. C qRT-PCR analysis for the mRNA expression of PRPH and TUJ1. D GFAP+ and S100B+ Schwann cells were detected by immunofluorescence. Scale bar, 50 μm. E The ratio of GFAP+ and S100B+ Schwann cells were counted and compared. F qRT-PCR analysis for the mRNA expression of GFAP and S100B. n = 3 biological replicates. Columns represent the mean ± SD. *p < 0.05, **p < 0.01.

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