Fig. 8: DUSP6 regulated the osteoclastogenesis via SMAD2 signaling. | Cell Death & Disease

Fig. 8: DUSP6 regulated the osteoclastogenesis via SMAD2 signaling.

From: DUSP6 expression is associated with osteoporosis through the regulation of osteoclast differentiation via ERK2/Smad2 signaling

Fig. 8

RANKL-induced osteoclast differentiation was established. A BMMs were transfected with SiControl (SiCtrl) and SiDUSP6. And the phosphorylation of SMAD2 was examined for the indicated time point. B Immunofluorescence assay was carried out to further explore the expression of P-SMAD2.F-actin ring formation was carried out to define the mature osteoclast. C Immunoprecipitation assay was carried out to clarify the connection between DUSP6 and P-SMAD2. D BMMs were transfected with SiCtrl and SiDUSP6. Immunofluorescence assay was carried out to detect the NFATC1 expression in the nucleus. E BMMs were transfected with SiControl (SiCtrl) and SiDUSP6. And they were then treated with the P-SMAD2 inhibitor SB31542 to further confirm the effect of P-SMAD2 in DUSP6-mediated osteoclastogenesis. TRAP staining (E) and quantitative analysis were carried out (F) to determine the osteoclast differentiation. Data in all bar graphs are expressed as mean ± SD (n = 3). Original scale bars: 200 μm.*P < 0.05, #P < 0.01.

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