Fig. 1: CHFR interacts and negatively correlates with ZEB1.
From: CHFR regulates chemoresistance in triple-negative breast cancer through destabilizing ZEB1
a Mass spectrometry identified a partial list of ZEB1 associated proteins. b, c Transiently transfected SFB-ZEB1, MYC-CHFR, and MYC-GFP into HEK293T cells, followed by pulldown with Sepharose beads (s-S beads) (b) or MYC beads (c) and immunoblotting with the antibodies indicated. d The schematic diagram of FHA, RING, and CRD domains of CHFR. Full-length CHFR (FL), CHFR∆FHA, CHFR∆RING, and CHFR∆CRD were transiently transfected into HEK293T cells, followed by pulldown with Sepharose beads (s-S beads) or MYC beads and immunoblotting with the antibodies indicated. e Immunoblotting of ZEB1, CHFR, and β-actin in non-TNBC cell MCF7 and TNBC cells LM2, SUM159, BT549, and MDA-MB-231. f SUM159 cells were treated with 0.5 µg ml−1 nocodazole overnight, the mitotic cells were “shaken off,” and then released into normal medium. Samples were collected at 0 and 5 h after releasing and analyzed by western blotting with ZEB1, CHFR, and β-actin. Cell cycle distribution was marked by Cyclin A and p-H3 (S10).
