Fig. 3: CHFR regulates chemosensitivity in TNBC cells.

a CHFR was stably transduced in LM2 cells and immunoblotted with the CHFR and β-actin antibodies. b–d LM2 cells stably transformed with mock or CHFR were seeded in 96-well plates and treated with 5-FU, DOX, or paclitaxel as indicated and then fixed with 10% methanol and stained with 1:1000 crystal violet. e, f CHFR was stably transduced in SUM159 and LM2 cells and immunoblotted with the CHFR, FASN, and β-actin antibodies. g ZEB1 was stably knocked down in LM2 cells and immunoblotted with the ZEB1, FASN, and β-actin antibodies. h CHFR and ZEB1 were transfected as indicated and immunoblotted with the MYC, FLAG, FASN, and β-actin antibodies. i Full-length MYC-CHFR and MYC-CHFR∆FHA, MYC-CHFR∆RING, and MYC-CHFR∆CRD mutants were transiently transfected in SUM159 cells and immunoblotted with the MYC, FASN and β-actin antibodies. Significance of Mock versus CHFR is shown. n = 3 wells per group. Data in b–d are the mean of biological replicates from a representative experiment, and error bars indicate s.e.m. Statistical significance was determined by a two-tailed, unpaired Student’s t test. The experiments were repeated three times.