Fig. 2: ZNF24 was the critical target of ZNFTR in PC.
From: LncRNA ZNFTR functions as an inhibitor in pancreatic cancer by modulating ATF3/ZNF24/VEGFA pathway
A The schematic diagram showed the position relationship between ZNFTR and ZNF24. The data were searched from the UCSC Genome Browser. B After transfected with siNC or siZNFTR #1/2, and pcDNA-Vector or pcDNA-ZNFTR in BxPC-3 and PANC-1 cells, the expression of ZNF24 both at mRNA and protein levels was detected by qRT-PCR and western blot, respectively. C After co-transfected with pcDNA-ZNFTR and siNC/siZNF24 or co-transfected with siZNFTR and pcDNA-vector/pcDNA-ZNF24 in PANC-1 cells, the expression of ZNF24 both at mRNA and protein levels was measured by qRT-PCR and western blot, respectively. D After co-transfected with siZNFTR and pcDNA-vector/pcDNA-ZNF24 or co-transfected with pcDNA-ZNFTR and siNC/siZNF24 in PANC-1 cells, the proliferation of transfected PANC-1 cells was analyzed via CCK-8 assay for 5 days. E, F To assess the invasive and migrative abilities of PANC-1 cells, co-transfected with pcDNA-ZNFTR and siNC/siZNF24 or co-transfected with siZNFTR and pcDNA-vector/pcDNA-ZNF24, Transwell assay (E) and Wound healing (F) were conducted, respectively. The histograms were showed as quantized relative invaded cells or wound size. Five fields were selected randomly and averaged. All data were revealed as means ± standard deviation (SD) for no less than three independent experiments. Significant P values showed as *P < 0.05, **P < 0.01, and ***P < 0.001.
