Fig. 6: ZNFTR was downregulated by the complex of HIF1-α and HDAC1 under hypoxia conditions.

A The relative expression of ZNFTR under normoxia, hypoxia (1% O₂), and CoCl₂ (100 μM) for 24 h were analyzed by qRT-PCR. The expression of ZNFTR was analyzed after transfected siNC or siHIF-1α #1/2 during hypoxia by qRT-PCR. B The expression of ZNFTR in PANC-1 cells under normoxia, hypoxia, or hypoxia and transfected with siHIF-1α #1 conditions were detected via RNA-FISH assay. DAPI was used to stain the nucleus. Scale bar: 50 µm. C The schematic diagram showed the predicted binding site between HIF-1α and the promoter of ZNFTR. D ChIP assay was utilized to analyze the binding between HIF-1α and the promoter of ZNFTR under normoxia, hypoxia, or hypoxia and transfected with siHIF-1α #1. E After transfected with a vector containing wild type (WT) or mutant binding site (MUT) of ZNFTR promoter, transcription activity of the ZNFTR was assessed via luciferase reporter assay under normoxia, hypoxia, or hypoxia and transfected with siHIF-1α #1 in PANC-1 cells. F The Co-IP assay was conducted to demonstrate the interaction between HIF-1α and HDAC1 during normoxia and hypoxia. G ChIP assay was utilized to analyze the binding interaction between HDAC1 and promoter of ZNFTR under normoxia and hypoxia conditions. H After PANC-1 cells transfected with a vector containing wild type (WT) or mutant binding site (MUT) of ZNFTR promoter, transcription activity of ZNFTR was assessed via luciferase reporter assay under normoxia, hypoxia, hypoxia with TSA (10 μM) for 3 h, or hypoxia with siHDAC1 condition. I The relative expression of ZNFTR on DMSO (150 μM) for 3 h or TSA (10 μM) for 3 h, and transfected with siNC or siHDAC1 during normoxia and hypoxia were detected by qRT-PCR. All data were revealed as means ± standard deviation (SD) for no less than three independent experiments. Significant P values showed as *P < 0.05 and **P < 0.01. n.s. means the difference was not significant.