Fig. 3: ZFAS1 acted as a miR-497-5p sponge in PC. | Cell Death & Disease

Fig. 3: ZFAS1 acted as a miR-497-5p sponge in PC.

From: Long non-coding RNA ZFAS1 promotes pancreatic cancer proliferation and metastasis by sponging miR-497-5p to regulate HMGA2 expression

Fig. 3

A FISH showed that ZFAS1 was mainly distributed in both cytoplasm and nucleus in PC cells. Scale bar, 25 μm. B qRT-PCR detected the ZFAS1 level in cytoplasm and nuclear. C The binding sites of miR-497-5p on the ZFAS1. D Luciferase activity of PC cells transfected with ZFAS1-WT or ZFAS1-MUT reporter together with miR-497-5p or NC. E MS2-RIP followed by miRNA qRT-PCR to detect endogenous miR-497-5p associated with the MS2-tagged ZFAS1 in PC cells. F PC cells were transfected with biotin-labelled miR-497-5p, and assayed by biotin-pull-down. ZFAS1 levels were analysed by qRT-PCR. G The expression of miR-497-5p in PC cells following transfection with ZFAS1 siRNA or NC. H The correlation of miR-497-5p and ZFAS1 expression in 26 PC tissues was negative. I TCGA dataset revealed a negative correlation between miR-497-5p and ZFAS1 expression in PC tissues. *P < 0.05, **P < 0.01, ***P < 0.001.

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