Fig. 2: The effects of MTDH on apoptosis and migration of Gem-treated breast cancer cells. | Cell Death & Disease

Fig. 2: The effects of MTDH on apoptosis and migration of Gem-treated breast cancer cells.

From: MiR-9-3p regulates the biological functions and drug resistance of gemcitabine-treated breast cancer cells and affects tumor growth through targeting MTDH

Fig. 2

A Flow cytometry was used to determine the effect of silencing MTDH expression on apoptosis of MCF7 cells treated by Gem (n = 3, *P < 0.05, **P < 0.01, vs. siNC; #P < 0.05, vs. siNC + Gem; ^P < 0.05, ^^P < 0.01, vs. siMTDH). B Flow cytometry was used to determine the effect of overexpressed MTDH on apoptosis of HCC1806 cells treated by Gem (n = 3, +P < 0.05, +++P < 0.001, vs. NC; ▲▲P < 0.01, vs. NC + Gem; △△P < 0.01, vs. MTDH). C The expression of cleaved caspase-3 and MTDH were detected by Western blot in MCF7 cells treated by siMTDH and Gem (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, vs. siNC; ###P < 0.001, vs. siNC + Gem; ^P < 0.05, ^^^P < 0.001, vs. siMTDH). D The expression of cleaved caspase-3 and MTDH were detected by Western blot in HCC1806 cells treated by MTDH and Gem (n = 3, +P < 0.05, +++P < 0.001, vs. NC; ▲▲▲P < 0.001, vs. NC + Gem; △P < 0.05, vs. MTDH). E Wound wound healing assay was used to detect MCF7 cell migration. (n = 3, **P < 0.01, ***P < 0.001, vs. siNC; #P < 0.05, vs. siNC + Gem; ^^P < 0.01, vs. siMTDH). F Wound scratch assay was used to detect HCC1806 cell migration (n = 3, +++P < 0.001, vs. NC; ▲▲P < 0.01, vs. NC + Gem; △△P < 0.01, vs. MTDH). MTDH Metadherin, NC negative control.

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