Fig. 5: TGM2 expression is correlated with HOTAIRM1 expression. | Cell Death & Disease

Fig. 5: TGM2 expression is correlated with HOTAIRM1 expression.

From: The long non-coding RNA HOTAIRM1 promotes tumor aggressiveness and radiotherapy resistance in glioblastoma

Fig. 5

A qRT-PCR for TGM2 expression was performed in control and stable HOTAIRM1 knock-down LN-229, U87MG, SF126, and LN-18 cell lines. White bar indicates the results of the respective control cells set to 100%. Note that HOTAIRM1 knock-down significantly reduces TGM2 mRNA levels. B–D Western blotting analysis of TGM2 protein expression in control versus stable HOTAIRM1 knock-down LN-229 (B) and U87MG (C) cell lines. Shown are three independent experiments. Beta-actin (ActinB) was used as a loading control. D Quantification of TGM2 protein expression by western blotting analysis in control versus stable HOTAIRM1 knock-down LN-229 and U87MG cell lines. White bar indicates the results of the respective control cells set to 100%. Two-way ANOVA was used for statistical calculation for qRT-PCR and Student’s t test was used for statistical analysis. Control: non-target shRNA; KD: shRNA-mediated HOTAIRM1 knock-down; mean ± SEM, ***p < 0.001, **p < 0.01, *p < 0.05. n = 3.

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