Fig. 8: PAR1 inhibition suppresses EMT in CRC cells. | Cell Death & Disease

Fig. 8: PAR1 inhibition suppresses EMT in CRC cells.

From: KLK8 promotes the proliferation and metastasis of colorectal cancer via the activation of EMT associated with PAR1

Fig. 8

A Western blotting analysis showed that PAR1 inhibition decreased the expression levels of the epithelial cell markers E-cadherin and occludin and increased the mesenchymal markers N-cadherin and vimentin in RKO and SW480 cells compared with the Lv-KLK8 group. B, C Quantification of the protein expression of E-cadherin, N-cadherin, vimentin and occludin represented in (A). D, E Immunofluorescence of E-cadherin and vimentin in RKO (D) and SW480 cells (E) treated with PAR1 antagonists SCH79797 and PAR2 antagonist FSLLRY-NH2. All of the data are presented as the mean ± SEM from three independent experiments. **P < 0.01 vs. Lv-control; ##P < 0.01 vs. Lv-KLK8.

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