Fig. 3: SRGN inhibition suppresses MGC formation and tumor growth of GCTB in vivo.
From: Serglycin induces osteoclastogenesis and promotes tumor growth in giant cell tumor of bone
A H&E, IHC, and TRAP staining of bone sections after intratibial injection of GCTB-19 cells with SRGN knockdown in NOD/SCID mice. B Quantification of TRAP-positive cells in bone sections. C Representative images of bioluminescent imaging (BLI) analyses of the whole bodies and hind limbs of the mice, and micro-CT analyses for bone destruction of hind limbs. Arrows point to osteolytic areas in the legs. D Weekly BLI quantitation of tumor burden of the mice (n = 6 mice per group). E Ex vivo BLI quantitation of tumor burden in hind limbs. F Micro-CT quantification of relative bone volumes of the mice. BV/TV bone volume/total volume. Scale bar, 100 μm. P values were obtained by Mann−Whitney U test (D, E) and two-tailed unpaired t test (A, F). Box plots display values of minimum, first quartile, median, third quartile, and maximum. Bar graphs are shown as mean ± s.d.
