Fig. 5: USP14 and USP22 co-mediated the induction of AR-V7 degradation and suppression of CRPC induced by nobiletin.
From: Selective degradation of AR-V7 to overcome castration resistance of prostate cancer
A Western blot of AR-V7 and USP14 in 22Rv1 cells transfected with two pairs of USP14 or control siRNAs for 48 h. B Western blot of AR-V7, Flag, and USP14 in 22Rv1 cells transfected with Flag-USP14 plasmids or control plasmids for 48 h. C Western blot of AR-V7 and USP22 in 22Rv1 cells transfected with two pairs of USP22 or control siRNAs for 48 h. D Western blot of AR-V7, His, and USP22 in 22Rv1 cells transfected with His-USP22 plasmids or control plasmids for 48 h. E Western blot of AR and AR-V7 protein level in 22Rv1 cells transfected with USP22 siRNAs or scramble siRNAs for 48 h and then exposed to CHX. F Quantitative data of E are shown. Mean ± SD (n = 3). G Western blot of AR and AR-V7 protein level in 22Rv1 cells transfected with His-USP22 plasmids or control plasmids for 48 h and then exposed to CHX. H Quantitative data of G are shown. Mean ± SD (n = 3). I Western blot of AR-V7, Flag, and USP14 protein level in 22Rv1 cells transfected with Flag-USP14 plasmids or control plasmids for 48 h and then exposed to nobiletin for 24 h. J CCK8 assays were performed in 22Rv1 cells transfected with Flag-USP14 plasmids or control plasmids for 24 h and then exposed to nobiletin for 48 h. Mean ± SD (n = 3). K Western blot of AR-V7, His, and USP22 protein level in 22Rv1 cells transfected with His-USP22 plasmids or control plasmids for 48 h and then exposed to nobiletin for 24 h. L CCK8 assays were performed in 22Rv1 cells transfected with His-USP22 plasmids or control plasmids for 24 h and then exposed to nobiletin for 48 h. Mean ± SD (n = 3).
