Fig. 5: Anti-TNFSF10 treatment modulated retinal expression of TNFSF10 and its TNFRSF10B receptor in 3xTg-AD mice. | Cell Death & Disease

Fig. 5: Anti-TNFSF10 treatment modulated retinal expression of TNFSF10 and its TNFRSF10B receptor in 3xTg-AD mice.

From: Targeting the miRNA-155/TNFSF10 network restrains inflammatory response in the retina in a mouse model of Alzheimer’s disease

Fig. 5

A Immunoblots of retinal lysates for the expression of TNFRSF10B and TNFSF10 proteins. B Densitometric analysis of western blots. Data are expressed as mean ± standard deviation. One-way ANOVA and post-hoc Tukey’s multiple comparisons test were used for statistical analysis. *p < 0.05. N = 5 animals; 5 independent retinal samples, 2 pooled retinas per sample in each group. C Immunohistochemical staining for TNFSF10 and its receptor TNFRSF10B in the retina of WT and 3xTg-AD mice, treated either with vehicle or anti-TNFSF10 antibody. Original magnification, x63. Scale bar = 10 µm. D Densitometric analysis of the TNFRSF10B and TNFSF10 immunofluorescence signal in the RPE and OPL retinal layers. Data are expressed as mean ± standard deviation. One-way ANOVA and post-hoc Tukey’s multiple comparisons test were used for statistical analysis. *p < 0.05. N = 5 animals; 5 independent retinal samples per group. For each retinal section, 14 optical fields were analyzed.

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