Fig. 6: Anti-TNFSF10 treatment inhibited pro-inflammatory microglia activation in the outer-plexiform and in the RPE layers of 3xTg-AD mouse retina. | Cell Death & Disease

Fig. 6: Anti-TNFSF10 treatment inhibited pro-inflammatory microglia activation in the outer-plexiform and in the RPE layers of 3xTg-AD mouse retina.

From: Targeting the miRNA-155/TNFSF10 network restrains inflammatory response in the retina in a mouse model of Alzheimer’s disease

Fig. 6

A Western blots for TNF-α, Ιba-1 and IL-10 protein expression in the retinas of 3xTg-AD mice, following chronic treatment with an anti-TNFSF10 monoclonal antibody or vehicle. B Densitometric analysis of western blots. Data are expressed as mean ± standard deviation. One-way ANOVA and post-hoc Tukey’s multiple comparisons test were used for statistical analysis. *p < 0.05. N = 5 animals; 5 independent retinal samples, 2 pooled retinas per sample in each group. C Immunohistochemical staining for TNF-α, Iba-1 in the retina of 3xTg-AD mice, treated with either vehicle or anti-TNFSF10 antibody. Original magnification, x63. Scale bar = 10 µm. D Immunohistochemical staining for Iba-1, IL-10 in the retina of WT and 3xTg-AD mice, treated with either vehicle or anti-TNFSF10 antibody. Original magnification, x63. Scale bar = 10 µm. E Densitometric analysis of the Iba-1, and TNF-α immunofluorescence signal in the RPE and OPL retinal layers. F Densitometric analysis of the Iba-1, and IL-10 immunofluorescence in the RPE and OPL retinal layers. Data are expressed as mean ± standard deviation. One-way ANOVA and post-hoc Tukey’s multiple comparisons test were used for statistical analysis. *p < 0.05. N = 5 animals; 5 independent retinal samples per group. For each retinal section, 14 optical fields were analyzed.

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