Fig. 7: Anti-TNFSF10 treatment inhibited astrogliosis in the outer-plexiform and in the RPE layers of the 3xTg-AD mouse retina. | Cell Death & Disease

Fig. 7: Anti-TNFSF10 treatment inhibited astrogliosis in the outer-plexiform and in the RPE layers of the 3xTg-AD mouse retina.

From: Targeting the miRNA-155/TNFSF10 network restrains inflammatory response in the retina in a mouse model of Alzheimer’s disease

Fig. 7

A Immunohistochemical staining for GFAP, COX2 in the retina of WT and 3xTg-AD mice treated with anti-TNFSF10 or vehicle. Original magnification, x63. Scale bar = 10 µm. B Densitometric analysis of the GFAP and COX2 immunofluorescence signal in the RPE and OPL retinal layers. One-way ANOVA and post-hoc Tukey’s multiple comparisons test were used for statistical analysis. * p < 0.05. N = 5 animals; 5 independent retinal samples per group. For each retinal section, 14 optical fields were analyzed. C Western blot images for GFAP, COX-2 protein expression in the retina of mice following chronic treatment with an anti-TNFSF10 monoclonal antibody or vehicle. D Densitometric analysis of western blots. Data are expressed as mean ± standard deviation. One-way ANOVA and post-hoc Tukey’s multiple comparisons test were used to determine statistical significance. *p < 0.05. N = 5 animals; 5 independent retinal samples, 2 pooled retinas per sample in each group.

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