Fig. 4: PPP6C knockout in mouse Sertoli cells by AMH-Cre results in the loss of germ cells.

A MVH (green) immunofluorescence analysis of the Ppp6cWT and Ppp6ccKO mice showed that the amount of the germ cells were decreased Scale bar: (left) 50 μm, (right) 20 μm. (B) SOX9 (green) immunofluorescence analysis of the Ppp6cWT and Ppp6ccKO mice showed that the amount and location of the Sertoli cells were similar. Scale bar: (left) 50 μm, (right) 20 μm. Nuclei are stained with DAPI. At least 3 mice of each genotype were used for analysis.