Fig. 8: PPP6C directly dephosphorylates β-catenin. | Cell Death & Disease

Fig. 8: PPP6C directly dephosphorylates β-catenin.

From: Specific deletion of protein phosphatase 6 catalytic subunit in Sertoli cells leads to disruption of spermatogenesis

Fig. 8

A Western blotting analysis of p-β-cateninS552 protein in Ppp6cWT and Ppp6ccKO Sertoli cells of mice. β-actin was detected as an internal control. At least 3 mice of each genotype were used for analysis. B A schematic diagram of in vitro phosphatase assays which were performed by using purified PPP6C (WT or D84N) and CTNNB1. C PPP6C (WT or D84N) was incubated with CTNNB1 to perform the in vitro phosphatase assays. Immunoblotting of the assay products exhibited an effective elimination of phospho-β-catenin (S552) by PPP6C, but not by the phosphatase-dead PPP6C (D84N). D β-Catenin immunofluorescence of Ppp6cWT and Ppp6ccKO Sertoli cells of mice. Scale bar: (top) 50 μm, (bottom) 10 μm.

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