Fig. 1: Ass1 expression increases during regeneration and upon loss of Apc.
From: Epithelial argininosuccinate synthetase is dispensable for intestinal regeneration and tumorigenesis
A Schematic overview of the mouse model. B Heatmap showing the expression of genes in the KEGG geneset ‘Arginine biosynthesis’, from RNA isolated from the intestines of irradiated mice at t = 0, 24, 48, and 96 h (n = 10 mice per time point). C qRT-PCR for Ass1 on intestinal lysates from the same experiment (n = 5 mice per time point), Actb is used as a reference gene. D Immunohistochemistry for ASS1 in small intestine at t = 0 and t = 96 h after irradiation. Micrographs show representative images. Scale bar represents 25 µm. E qRT-PCR for Ass1 in murine adenoma-to-carcinoma sequence organoids relative to non-induced control organoid (relative to Actb/Ppia), A = Apc−/−, K = KrasG12D/+, S = Smad4 shRNA, P = Trp53−/− (for single knockout) or Trp53 shRNA in combination with AKS. *p < 0.05, **p < 0.01 by student’s t-test. F Western blot for ASS1 and beta-actin in wild type (WT; from C57BL/6 mice), VillinCreERT2 Apcfl/fl and VillinCreERT2 Apc−/− organoids. G Immunohistochemistry for ASS1 in VillinCreERT2 Apcfl/fl and VillinCreERT2 Apc−/− organoids. Micrographs show representative images. Scale bar represents 50 µm. H Heatmap showing qRT-PCR results for various urea cycle and creatine synthesis, glutamate and proline metabolism, polyamine synthesis, and arginine transporter genes.
