Fig. 3: Loss of Apc leads to altered amino acid consumption and synthesis. | Cell Death & Disease

Fig. 3: Loss of Apc leads to altered amino acid consumption and synthesis.

From: Epithelial argininosuccinate synthetase is dispensable for intestinal regeneration and tumorigenesis

Fig. 3

A Amino acid concentrations as measured by HPLC in supernatant of wild-type, VillinCreERT2 Apcfl/fl, and VillinCreERT2 Apc−/− organoids. B schematic overview of labeling. C LC-MS measurement of intracellular M4 citrulline, M4 arginine, and M4 argininosuccinate, in wild-type and VillinCreERT2 Apc−/− organoids after incubation with labeled M4 citrulline (13C1;3,3,4-D3), and ratios between M3/M4-labeled citrulline and arginine. *p < 0.05 by student’s t-test.

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