Fig. 8: Plasma glutamine levels and expression of urea-cycle enzymes in the liver, might compensate for intestinal loss of Ass1.

A qRT-PCRs for indicated genes in liver tissue from VillinCre^ERT2 Ass1^wt/wt and VillinCre^ERT2 Ass1^−/− mice 96 h after 14 Gy irradiation. Actb was used as a reference gene. ^**p < 0.01 by Student’s t-test. B Plasma levels of indicated amino acids measured by HPLC from VillinCre^ERT2 Ass1^wt/wt and VillinCre^ERT2 Ass1^−/− mice 96 h after 14 Gy irradiation. ^*p < 0.05 by Student’s t-test. C qRT-PCRs for indicated genes in liver tissue from VillinCre^ERT2 Ass1^wt/wt Apc^wt/wt, VillinCre^ERT2 Ass1^wt/wt Apc^−/−, and VillinCre^ERT2 Ass1^−/− Apc^−/− mice, 5 days after induction. Actb was used as a reference gene. ^*p < 0.05 by one-way ANOVA with Tukey’s multiple comparisons test.