Fig. 3: USP7 is specifically required for the expression of SMAD3 in H1299 cells.
From: USP7 facilitates SMAD3 autoregulation to repress cancer progression in p53-deficient lung cancer
A Heatmaps of RNA-seq results showing the relative expression levels of genes involved in TGFβ-signal pathway in wildtype (WT) and USP7 KO (HKO_E2 and HKO_E3) H1299 lines. Two biological repeats for each line and rank in wildtype SE identification are shown. B RT-qPCR assays showing the relative expression levels of indicated genes in wildtype (WT) or USP7 KO (HKO_E2 and HKO_E3) H1299 cell lines. Means ± SD from three biological experiments are shown. Student’s t-test, ***P < 0.001. C Immunoblots of SMAD3 and SMAD4 in the lysates of wildtype (WT) or USP7 KO (HKO_E2 and HKO_E3) H1299 cell lines. β-actin as an internal control. Asterisk indicates a nonspecific signal. D GSEA plot showing the enrichment of TGFβ-responsive genes in H1299 wildtype (WT) relative to USP7 KO (HKO_E2) cells.
