Fig. 5: The deubiquitinase activity of USP7 is required for USP7-mediated transcriptional activation of SMAD3.
From: USP7 facilitates SMAD3 autoregulation to repress cancer progression in p53-deficient lung cancer
A Luciferase reporter assays showing the relative enhancer activities of SMAD3 SE constituents in H1299 wildtype and USP7 KO cells without or with ectopically expressed USP7 wildtype (WT) or deubiquitinase-deficient CS mutant. B, C The relative mRNA (B) or protein (C) levels of endogenous SMAD3 were determined by RT-qPCR or immunoblotting assays, respectively, in H1299 cells described in (A). Mean ± SD from three independent experiments. Student’s t-test, ***p < .001. Asterisks indicate nonspecific signals.
