Fig. 5: miR-138-5p regulates the inflammatory response by targeting NLRP3. | Cell Death & Disease

Fig. 5: miR-138-5p regulates the inflammatory response by targeting NLRP3.

From: Suppression of lncRNA NLRP3 inhibits NLRP3-triggered inflammatory responses in early acute lung injury

Fig. 5

A The predicted miR-138-5p-binding sites in the NLRP3 mRNA 3′-UTR. B A firefly luciferase reporter containing either wild-type or mutant NLRP3 was cotransfected into NR8383 AM cells with miR-138-5p mimics NC or miR-138-5p mimics. qRT-PCR assays were used to analyse the mRNA expression of C miR-138-5p, D NLRP3, E Caspase-1, F IL-18, and G IL-1β in the NR8383 AM cells (n = 6). β-Actin was used as a reference gene. H, I ELISA was used to analyse the IL-1β and IL-18 levels in the culture supernatants. J Western blotting assay of the protein expression levels of NLRP3 and Caspase-1. K, L Cell apoptosis was determined by Hoechst 33342 and PI dual staining assays (K) and counted (L). The data are presented as mean ± SE (n = 6). *P < 0.05; **P < 0.01; ***P < 0.001; NS, no statistically significant difference.

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