Fig. 2: Effects of metformin on renal oxidative stress and renal interstitial fibrosis in HFD/STZ induced diabetic mice. | Cell Death & Disease

Fig. 2: Effects of metformin on renal oxidative stress and renal interstitial fibrosis in HFD/STZ induced diabetic mice.

From: AMPK agonist alleviate renal tubulointerstitial fibrosis via activating mitophagy in high fat and streptozotocin induced diabetic mice

Fig. 2

A IHC analysis of 8-OHdG (magnification ×400, upper panel) and DHE staining (lower panel) in mouse renal tissue of three groups (magnification ×200). B, C Bar graphs representing quantification of tissues stained with 8-OHdG (B) and DHE (C), *P < 0.05 vs. control groups, #P < 0.05 vs. STZ + HFD groups, n = 3. D Western blot analysis of FN (upper panel) and Col-1(middle panel) protein expression in the renal tissue of three groups. E IF analysis of FN (upper panel) and Col-1 (middle panel) in mouse renal tissue of three groups (magnification ×400), renal tissue sections are stained with Masson (lower panel, magnification ×400). F, G Semiquantification of IF staining for FN (F) and Col-1 (G). H–I Densitometric analyses of the Western blotting results, FN to β-actin (H), Col-1 to β-actin (I). Values are presented as the mean ± SD, *P < 0.05 vs. control group, #P < 0.05 vs. STZ + HFD group, n = 3.

Back to article page