Fig. 4: Effects of metformin on mitophagy dysfunction in renal tubular cell of HFD/STZ induced diabetic mice. | Cell Death & Disease

Fig. 4: Effects of metformin on mitophagy dysfunction in renal tubular cell of HFD/STZ induced diabetic mice.

From: AMPK agonist alleviate renal tubulointerstitial fibrosis via activating mitophagy in high fat and streptozotocin induced diabetic mice

Fig. 4

A EM analysis showed obvious mitochondrial morphological changes in diabetic mice renal cells, such as mitochondria swelling and fragmentation, these changes were partially reversed by metformin therapy. In addition, metformin treatment group exhibited conspicuous mitochondrial autophagosome compared with the diabetic mice group (a–c, magnification ×10,000, upper panel; d–f, magnification ×20,000, bottom panel). B Renal IHC staining with anti-LC3II antibody (upper panel) and anti-Pink1 antibody (lower panel) (magnification ×400). C Western blot analysis of P62 (upper panel), Pink1, Parkin, Atg5 (middle panels), and LC3II (bottom panel) protein expression in mitochondria (left panels) and cytoplasm (right panels). D–H Densitometric analyses of the western blotting results, P62 to CoxIV or P62 to GAPDH (D), Pink1 to CoxIV or Pink1 to GAPDH (E), Parkin to CoxIV or Parkin to GAPDH (F), Atg5 to CoxIV or Atg5 to GAPDH (G), LC3II to CoxIV or LC3II to GAPDH (H). Values are presented as the mean ± SD, *P < 0.05 vs. control group, #P < 0.05 vs. STZ + HFD group, n = 3.

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