Fig. 6: Metformin treatment regulated FN, Col-1, NLRP3, p-AMPK expression, and mitochondrial ROS generation in HK-2 cells exposed to HG/HFA conditions. | Cell Death & Disease

Fig. 6: Metformin treatment regulated FN, Col-1, NLRP3, p-AMPK expression, and mitochondrial ROS generation in HK-2 cells exposed to HG/HFA conditions.

From: AMPK agonist alleviate renal tubulointerstitial fibrosis via activating mitophagy in high fat and streptozotocin induced diabetic mice

Fig. 6

A Western blot analysis of Pink1 protein expression in the HK-2 cells of control and Pink1 siRNA group. B Densitometric analyses of WB results, bar graph represents the ratio of Pink1 to β-actin, *P < 0.05 vs. control groups, n = 3. C Western blot analysis of FN (upper panel), Col-1, NLRP3, p-AMPK (middle panel), and AMPK (bottom panel) protein expression in the HK-2 cells of various groups. D–H Each bar graph represents the densitometric analyses of FN to β-actin (D), Col-1 to β-actin (E), NLRP3 to β-actin (F), p-AMPK to β-actin (G), AMPK to β-actin (H). J MitoSOX Red staining represented of mitochondrial ROS levels in HK-2 cells of various groups (magnification ×630). I Quantification of mitochondrial ROS production as measured with MitoSox Red staining. Values are presented as the mean ± SD, *P < 0.05 vs. LG group, #P < 0.05 vs. HG + HFA group, **P < 0.05 vs. HG + HFA + Met group, ##P < 0.05 vs. HG + HFA + Met group, n = 3.

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