Fig. 7: Metformin activated mitophagy in HK-2 cells under HG/HFA conditions through p-AMPK-Pink1-Parkin pathway. | Cell Death & Disease

Fig. 7: Metformin activated mitophagy in HK-2 cells under HG/HFA conditions through p-AMPK-Pink1-Parkin pathway.

From: AMPK agonist alleviate renal tubulointerstitial fibrosis via activating mitophagy in high fat and streptozotocin induced diabetic mice

Fig. 7

A Laser-scanning confocal microscopy detection showed Pink1 expression and mitochondrial morphology in HK-2 cells exposed to HG/HFA conditions and pretreated with metformin, Pink1 siRNA or compond C (magnification ×630). B Quantification of IF staining for Pink1. C Semi-quantification for the co-localization of Pink1 and mitochondria. D Western blot analysis of P62 (upper panel), Pink1, Parkin, Atg5 (middle panels), and LC3II (bottom panel) protein expression in mitochondria (left panels) and cytoplasm (right panels) of HK-2 cells. E–I Densitometric analyses of the western blotting results, Pink1 to CoxIV or Pink1 to GAPDH (E), P62 to CoxIV or P62 to GAPDH (F), Parkin to CoxIV or Parkin to GAPDH (G), Atg5 to CoxIV or Atg5 to GAPDH (H), LC3II to CoxIV or LC3II to GAPDH (I). Values are presented as the mean ± SD, *P < 0.05 vs. LG group, #P < 0.05 vs. HG + HFA group, **P < 0.05 vs. HG + HFA + Met group, ##P < 0.05 vs. HG + HFA + Met group, n = 3.

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