Fig. 4: RANBP10 stabilized c-Myc protein by mediating its ubiquitination degradation.
From: RANBP10 promotes glioblastoma progression by regulating the FBXW7/c-Myc pathway
A, B Western blot analysis and RT-PCR analysis were performed to detect the expression of some G1 cell regulatory proteins and metastasis-related proteins in RANBP10-knockdown and control cells. C Proteins were harvested from the RANBP10-knockdown and control cells that had been treated with or without MG132 for 8 h. Western blot analysis was used to examine the protein level of c-Myc. D, E RANBP10 overexpression and control cells were treated with CHX (100 μg/ml) for the indicated times, and then were harvested and detect the c-Myc turnover rate through western blot analysis. F The indicated plasmids were transfected into GBM cells, and MG132 was added to the cells before harvested. The ubiquitinated c-Myc proteins were pulled down with anti-HA antibody and immunoblotted with ant-c-Myc antibody. The data were expressed as mean ± SD. Student’s t test was performed to analyzed significance. *P < 0.05, **P < 0.01, ***P < 0.001.
