Fig. 5: RANBP10 suppressed the transcription of FBXW7.
From: RANBP10 promotes glioblastoma progression by regulating the FBXW7/c-Myc pathway
A Western blot analysis was used to detect the protein expression of FBXW7 in RANBP10 downregulation and control cells. B RT-PCR analysis was used to detect the mRNA expression of FBXW7 in RANBP10 downregulation and control cells. C Dual-luciferase reporter assays were used to detect the promoter activity of FBXW7. D ChIP assay was performed by using Flag antibodies. IgG was used as the negative control. E Western blot analysis was performed to examine the protein expression of FBXW7 and c-Myc in the indicated cells. F, G MTT assays were used to assess the proliferative effects of FBXW7 downregulation in RANBP10-knockdown and control cells. H, I Transwell assays were used to assess the metastatic effects of FBXW7 downregulation in RANBP10-knockdown and control cells. Scale bar = 50 μm. The data were expressed as mean ± SD. Student’s t test was performed to analyzed significance. *P < 0.05, **P < 0.01, ***P < 0.001.
