Fig. 1: Identification of circUBE2Q2 in GC. | Cell Death & Disease

Fig. 1: Identification of circUBE2Q2 in GC.

From: Circular RNA UBE2Q2 promotes malignant progression of gastric cancer by regulating signal transducer and activator of transcription 3-mediated autophagy and glycolysis

Fig. 1

A Clustered heatmap showing the top 20 differentially expressed circRNAs in three paired human gastric cancer tissues relative to paired normal tissues. B qRT-PCR expression of circUBE2Q2 in GC tissues relative to matched normal tissues (n = 60). C Sanger-sequencing-validated circular structure of circUBE2Q2. The arrows indicate the splicing sites of circUBE2Q2. D qRT-PCR quantified the abundance of circUBE2Q2 and linear UBE2Q2 in MKN-45 and BGC-823 cells treated after RNase R treatment. E Agarose gel electrophoresis assay for products of linear and back-splicing amplified with convergent and divergent primers with and without RNase R treatment. F qRT-PCR analysis of circUBE2Q2 and UBE2Q2 after treatment with Actinomycin D at the specified time points. G qRT-PCR analysis of the cytoplasm and nucleus of circUBE2Q2, respectively. H Fluorescence in situ hybridization (FISH) for circUBE2Q2 localization, scale bar = 20 µm. Nuclei were stained blue with DAPI. Data are expressed as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.

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