Fig. 4: STAT3 is a downstream target of miR-370-3p. | Cell Death & Disease

Fig. 4: STAT3 is a downstream target of miR-370-3p.

From: Circular RNA UBE2Q2 promotes malignant progression of gastric cancer by regulating signal transducer and activator of transcription 3-mediated autophagy and glycolysis

Fig. 4

A Venn diagram detailing the exploration of miR-370-3p downstream targets. B Overall survival analysis based on STAT3 expression in TCGA GC patients. The optimal cutoff was calculated by X-tile software. C mRNA levels of STAT3 in GC tissues and paired normal tissues detected by qRT-PCR (n = 60). D Correlation of STAT3 and miR-370-3p expression in GC tissues and paired normal tissues (n = 60). E The expression of PACS1 and STAT3 after miR-370-3p silencing in MKN-45 cells measured by qRT-PCR. F A dual-luciferase reporter assay to determine the direct binding between miR-370-3p and STAT3 in MKN-45 cells. G Expression of STAT3 detected via immunochemistry analysis (IHC) on 12 paired GC tumor tissues and normal stomach tissues, scale bar = 20 µm. H Correlation of STAT3 and circUBE2Q2 expression in GC tissues (n = 60). I The expression of STAT3 in GC cells transfected with circUBE2Q2-specific siRNA measured by qRT-PCR. J The protein levels of STAT3 in GC cells transfected with circUBE2Q2-specific siRNA measured by western blottings. K Immunofluorescence staining for STAT3 in treated MKN-45 and BGC-823 cells, scale bar = 20 µm. Data are expressed as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.

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