Fig. 2: ITSN1-S contained a nuclear localization signal (NLS) in resides 306–312. | Cell Death & Disease

Fig. 2: ITSN1-S contained a nuclear localization signal (NLS) in resides 306–312.

From: Endocytic protein intersectin1-S shuttles into nucleus to suppress the DNA replication in breast cancer

Fig. 2

A Various fragments of ITSN1-S labeled with flag and HA tags were transfected into MDA-MB-231 cells. Top: domain structure of human ITSN1-S. Bottom: expression of exogenous fragments (EH1,2, CC, and 5SH3) were monitored by anti-HA and anti-flag antibodies in Western blot analysis, respectively. β-actin was used as a loading control. B Expression of exogenous fragments of ITSN1-S in the cytoplasm (Cyto) and nuclei (Nuc) of MDA-MB-231 cells were detected by anti-HA and anti-flag antibodies in Western blot analysis, respectively. C Localization of exogenous ITSN1-S fragments were detected by immunofluorescence with anti-HA (left panel) and anti-flag (right panel) antibodies in MDA-MB-231 cells, respectively. Scale bars, 25 μm. D Left: schematic presentation of the different NLS mutants of ITSN1-S. The mutated residues were shown in red. Right: expression of 3×flag-labeled mutated NLSs were detected by anti-flag antibody in Western blot analysis. β-actin was used as a loading control. E Localization of 3×flag-labeled mutated NLSs were detected by immunofluorescence with anti-flag antibody in MDA-MB-231 cells. Scale bars, 25 μm.

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