Fig. 6: GALNT4 can regulate apoptosis and directly binds to ASK1 to inhibit its oligomerization.
From: Role of GALNT4 in protecting against cardiac hypertrophy through ASK1 signaling pathway
A Knockdown of GALNT4 increases the expression of apoptotic proteins in vivo. B The overexpression of GALNT4 inhibits cardiomyocytes apoptosis. A, B **p < 0.01 vs. AdshRNA PBS or AdVector PBS, #p < 0.05 vs. AdshRNA PE or AdVector PE, ##p < 0.01 vs. AdshRNA PE or AdVector PE. C, D The expression of Bax and C-caspase 3 in vitro. For C-D, *p < 0.05 vs. WT sham or AAV9-Vector sham, **p < 0.01 vs. WT sham or AAV9-Vector sham, #p < 0.05 vs. WT TAC or AAV9-Vector TAC, ##p < 0.01 vs. WT TAC or AAV9-Vector TAC. E, F The interaction between GALNT4 and ASK1 in 293T cells. G The interaction between GALNT4 and ASK1 in NRCMs. H, I A GST pull down assay was used to verify the direct combination between GALNT4 and ASK1. J The effect of GALNT4 on the dimerization of ASK1 (1–678) in 293T cells.
