Fig. 1: miR-193b-3p is downregulated in psoriasis patients and M5-induced keratinocytes.

A Representative images showing the pathological morphology of skin tissues derived from psoriasis patients and healthy donors using H&E staining. Scale bar: 50 µm. B Expression levels of miR-193b-3p in skin tissues derived from healthy donor (CTL, n = 5) and psoriasis patient (Psoriasis, n = 6). mRNA levels were analyzed using quantitative real-time PCR (qRT-PCR). U6 was used as the internal control. Each bar represents mean ± SEM. ^*P < 0.05, compared with the indicated controls. C Correlation of miR-193b-3p expression with the PASI score of corresponding patients (n = 6). D Expression levels of miR-193b-3p in HaCaT cells in the presence or absence of M5 treatment. mRNA levels were analyzed using qRT-PCR. U6 was used as the internal control. Each bar represents mean ± SEM (n = 4). ^*P < 0.05, compared with the indicated controls. E Expression levels of miR-193b-3p in normal human keratinocyte cells (NHK cells) in the presence or absence of M5 treatment. mRNA levels were analyzed using qRT-PCR. U6 was used as the internal control. Each bar represents mean ± SEM (n = 4). ^*P < 0.05, compared with the indicated controls. F Representative images showing the skin lesions of Vaseline (CTL) and IMQ-treated mice (IMQ). G Expression levels of miR-193b-3p in the back skin of Vaseline (CTL) and IMQ-treated mice (IMQ). mRNA levels were analyzed using qRT-PCR. U6 was used as the internal control. Each bar represents mean ± SEM (n ≥ 4). ^**P < 0.01, compared with the indicated controls.