Fig. 2: Function of miR-193b-3p in M5-induced HaCaT cells.

A Expression levels of psoriasis-related genes and inflammatory genes in HaCaT cells transfected with miR-193b-3p mimics (miR-193)/control (miR-NC) in the presence or absence of M5 treatment. mRNA levels were analyzed using qRT-PCR. GAPDH was used as the internal control. Each bar represents mean ± SEM (n = 3). ^***P < 0.001, ^**P < 0.01, ^*P < 0.05, ^###P < 0.001, ^##P < 0.01, compared with the indicated controls. B Representative blots showing the levels of phospho-STAT3 (Tyr705), phospho-STAT3 (Ser727), phospho-NF-κB p65 (Ser311), IL-1β, etc. in HaCaT cells transfected with miR-193b-3p mimics (miR-193) or miR-193b-3p inhibitors (in-193) and their indicated CTLs (miR-NC/in-NC) in the presence or absence of M5 treatment. Protein levels were detected by western blotting. GAPDH was used as a protein loading control. C Expression levels of psoriasis-related genes and inflammatory genes in HaCaT cells transfected with miR-193b-3p inhibitors (in-193)/control (in-NC) in the presence or absence of M5 treatment. mRNA levels were analyzed using qRT-PCR. GAPDH was used as the internal control. Each bar represents mean ± SEM (n = 3). ^***P < 0.001, ^**P < 0.01, ^*P < 0.05, ^###P < 0.001, ^##P < 0.01, compared with the indicated controls.